Gold. Now the copper(II) undergoes reduction and is converted to copper(I). The biuret reagent is made up of hydrated copper sulfate, sodium hydroxide, and Rochelle salt (sodium-potassium tartrate). Biochem. It is based on the biuret reaction in which a peptide structure containing at least two peptide links produces a violet color when treated with alkaline copper sulfate. 193 (1951) 265 - 275. Since this test is not greatly disturbed by the presence of amino acids in the sample, it can be used to gauge the concentration of proteins in whole tissue samples. In the presence of peptides, a copper(II) ion forms mauve-colored coordination complexes in an alkaline solution. 5. Similarly, three amino acids linked together by two peptide bonds are called tripeptide, and as the chain elongates, it is called a polypeptide. Substances containing not less than two peptide linkages give this test. Biochem. Shrestha B (2002). The intensity of the color is directly proportional to the number of the peptide bonds present in the protein molecule that is reacting and also the number of the protein molecules present in the reaction system. The development of a purple coloured complex is a positive indicator for the biuret test. The biuret test, also known as Piotrowski's test, is a chemical test used for detecting the presence of peptide bonds. Formation of a chelate complex in the biuret test. The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. 2007, 84, 1676-1678. 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